Figure 5
From: FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in endometrial cancer
FOXA1 promotes proliferation of human EC cells by affecting AR-mediated transcription. A: Proliferation in MFE-296 cells transfected with NC or shFOXA1 was assessed by the colony-forming assay (Left) and further quantified in the number of colonies of triplicate experiments (Right). B: Proliferation in AN3CA cells transfected with NC or exFOXA1 was assessed by the colony-forming assay (Left) and further quantified in the number of colonies of triplicate experiments (Right). C: Assessment of proliferation by the MTT assay in MFE-296 cells transfected with NC or shFOXA1. D: Assessment of proliferation by the MTT assay in AN3CA cells transfected with NC or exFOXA1. E: Left: Colony-formation assay of untransfected MFE-296 cells (WT) and MFE-296 cells transfected with NC, shFOXA1, or shFOXA1 and exAR. Right: Graphical representation of the fold change in the number of colonies in untransfected MFE-296 cells (MFE-296) and MFE-296 cells transfected with NC (MFE-296/NC), shFOXA1 (MFE-296/shFOXA1), or shFOXA1 and exAR (MFE-296/shFOXA1 + exAR). F: Proliferation of MFE-296, MFE-296/NC, MFE-296/shFOXA1, or MFE-296/shFOXA1 + exAR cells was assessed by MTT assay. The right panel reiterates the data in the left panel at 72 h. *p < 0.05, **p < 0.01, ***p < 0.001 and NS p > 0.05.