Figure 3
From: FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in endometrial cancer
FOXA1 affects AR-mediated transcription. A: MFE-296 cells were treated with DHT (10−9 to 10−7 M) or vehicle (control) for 24 h. qRT-PCR was used to assess the levels of AR, XBP1, MYC, ZBTB16, and UHRF1 mRNA. The levels of each mRNA are shown relative to the level expressed in the vehicle sample. B: Quantification of AR, XBP1, MYC, ZBTB16, and UHRF1 mRNA by qRT-PCR in MFE-296 cells treated with 10−7 M DHT for 0–48 h. C: Western blotting analysis of AR in MFE-296 cells treated with vehicle, 10−7 M DHT, or 10−7 M DHT plus 10−6 M flutamide (DHT + FLU) for 24 h. β-actin was used as a loading control. D: MFE-296/NC and MFE-296/shFOXA1 cells were treated with 10−7 M DHT or vehicle for 24 h followed by qRT-PCR analysis of AR, XBP1, MYC, ZBTB16, and UHRF1 mRNA. E: AN3CA/NC and AN3CA/exFOXA1 cells were treated with 10−7 M DHT or vehicle for 24 h followed by qRT-PCR analysis of AR, XBP1, MYC, ZBTB16, and UHRF1 mRNA. F: Quantification of AR expression by qRT-PCR and western blotting in untransfected MFE-296 cells (MFE-296) and MFE-296 cells transfected with NC (MFE-296/NC) or exAR (MFE-296/exAR). G: Expression of XBP1, MYC, ZBTB16, and UHRF1 mRNA in untransfected MFE-296 cells and MFE-296 cells transfected with NC, shFOXA1, or shFOXA1 and exAR was measured by qRT-PCR. H: Quantification of AR expression by qRT-PCR and western blotting in untransfected AN3CA cells (AN3CA) and AN3CA cells transfected with NC (AN3CA/NC) or siAR (AN3CA/siAR). I: Expression of XBP1, MYC, ZBTB16, and UHRF1 mRNA in untransfected AN3CA cells and AN3CA cells transfected with NC, exFOXA1, or exFOXA1 and siAR was measured by qRT-PCR. *p < 0.05, **p < 0.01, ***p < 0.001, NS p > 0.05.