Skip to main content
Figure 2 | BMC Cancer

Figure 2

From: FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in endometrial cancer

Figure 2

FOXA1 affects the expression of AR in human EC cells. A: FOXA1 and AR expression in the indicated EC cell lines as determined were measured by western blotting (Left), and further quantified by densitometry of triplicate experiments (Right). β-actin was used as a loading control. B: Stable transfection of MFE-296 cells with negative control vector (MFE-296-NC) or shFOXA1 (MFE-296-shFOXA1). By comparing the cells in white light (the upper panels) with the cells in green fluorescence (the lower panels), the percentage of transfected/fluorescing cells was estimated at >85%. Magnification, ×400. C: Quantification of FOXA1 mRNA by qRT-PCR in untransfected MFE-296 (MFE-296), MFE-296 transfected with shRNA control plasmid (MFE-296/NC), and MFE-296 transfected with shFOXA1 (MFE-296/shFOXA1). D: Quantification of AR mRNA by qRT-PCR in MFE-296, MFE-296/NC, and MFE-296/shFOXA1 cells. E: FOXA1 and AR expression in MFE-296, MFE-296/NC and MFE-296/shFOXA1 cells were measured by western blotting (Left), and further quantified by densitometry of triplicate experiments (Right). F: Quantification of FOXA1 mRNA by qRT-PCR in untransfected AN3CA (AN3CA), AN3CA transfected with control plasmid (AN3CA/NC), and AN3CA transfected with FOXA1 expression plasmid (AN3CA/exFOXA1). G: Quantification of AR mRNA by qRT-PCR in AN3CA, AN3CA/NC, and AN3CA/exFOXA1 cells. H: AR and FOXA1 expression in AN3CA, AN3CA/NC and AN3CA/exFOXA1 cells were measured by western blotting (Left), and further quantified by densitometry of triplicate experiments (Right). *p < 0.05, **p < 0.01, NS p > 0.05 compared with NC.

Back to article page