Figure 4

MiRNAs deregulated in CS, FS and NS lung adenocarcinomas are associated with patient survival. Associations between miRNA expression and LUAC patient survival were assessed for miRNAs identified as deregulated in LUAC using a logrank, Mantel-Haenszel test. Survival analyses were performed independently for tumors from all smoking status groups, CS, FS, and NS patients. Numerous miRNAs commonly disrupted across CS, FS, and NS were significantly associated with LUAC patient survival, including miR-1 (A) and miR-153 (B) (B-H p <0.05) which were both connected to multiple lung cancer prognostic genes from Figure 3. MiR-138, which was preferentially underexpressed in CS tumors, was also significantly associated with CS LUAC patient outcome, with low expression associated with poor survival (C) (p <0.05). High expression of EZH2, a biologically validated target of miR-138, showed a significant association with poorer survival in CS LUAC patients (D) (p <0.05). OE, overexpressed. UE, underexpressed. NS, never smokers. CS, current smokers. FS, former smokers. B-H, Benjamini-Hochberg multiple-test-corrected.