Figure 2

Network interactions between deregulated lung tumor miRNAs and their predicted mRNA targets. MiRNAs specifically disrupted in CST, FST, or NST tumors were inputted into mirDIP to identify their predicted gene targets (i.e., mRNA transcripts predicted by at least six miRNA target prediction algorithms). The network of identified miRNA-mRNA interactions was then generated and visualized using NAViGaTOR. Only the most highly connected miRNAs were used to build the network. miRNAs specifically deregulated in CST, FST, and NST are indicated by blue, green, and red colored square nodes, respectively. Predicted mRNA targets are represented as circular nodes. Edges indicate miRNA-mRNA interactions, and are color-coded to match smoking group specificity of miRNA deregulation. Numerous target genes were shared by miRNAs specifically deregulated in CS, FS, and NS, as shown to the right of the miRNAs list in the centre. Conversely, targets unique to specific smoking groups are indicated to the left of the list. Predicted targets uniquely mapping to miRNAs disrupted in one smoking status group are represented by circles on the left; 1,162 mRNA targets were unique to miRNAs altered in CST, 927 to NST miRNAs, and 770 to FST miRNAs. Conversely, 1,399 mRNA-miRNA targets were shared between miRNAs altered uniquely in each smoking status group. MiR-532 was underexpressed in CST and overexpressed in NST. Gene Ontology terms associated with predicted target genes are indicated by target gene shading.