Effects of embelin on metastasis-mediating proteins. Western blot analyses of proteins involved in cancer cell migration. (A) MMP-2, MMP-9, VEGF and hnRNP-K decrease in embelin-treated MCF-7 cells. The pixel calculation of western blots by actin normalization was done using ImageJ software (NIH, MA). (B) Decrease in the expression of hnRNP-K, as detected by immunostaining. (C) Schematic representation of possible action of embelin on cancer cell metastasis as based on the present study.