Effects of embelin on proliferation and migration of breast cancer cells. (A) Expression of mortalin in mortalin-myc transduced metastatic cells detected by western blotting with anti-myc and anti-mortalin antibodies. (B) Morphology of embelin-treated control and mortalin-overexpressing metastatic cells indicative of growth arrest. (C) Viability of human breast cancer cells (MCF7 and MDA-MB-231) and their mortalin-overexpressing derivatives. The 4-parameter logistic curve is plotted using Prism 6 software (GraphPad Software, Inc., CA). (D) Colony forming efficacy and quantitation (E) from three independent experiments is shown. ***p < 0.001.