Skip to main content

Table 2 Electroblot esterase activity bands contain OPH

From: Identification of oxidized protein hydrolase as a potential prodrug target in prostate cancer

B and 2
Protein Identified P MW(D a)
N-acylaminoa cly-peptide hydrolase 3.710E-05 81172.77
Peptides Identified P MW(D a)
R.QVLLSEPEEAAALYR.G 1.230E-02 556.27
K.ALDVSASDDEIAR.L 5.072E-01 103.52
K.SFNLSALEK.H 8.916E-01 159.19
K.AESFFQTK.A 9.150E-01 159.21
R.VVFDSAQR.S 8.716E-01 113.36
R.QVLLSEPEEAAALYR.G 4.985E-05 439.67
B and 3
Protein Identified P MW(D a)
N-acylaminoacyl-peptide hydrolase 1.857E-07 81172.77
Peptides Identified P MW(D a)
R.QVLLSEPEEAAALYR.G 1.857E-07 1185.89
R.GSTGFGQDSILSLPGNVGHQDVK.D 3.389E-04 541.33
K.TPLLLML GQEDR.R 2.792E-05 1402.32
R.NVPVR.D 9.825E-01 91.84
B and 4
Protein Identified P MW(D a)
N-acylaminoacyl-peptide hydrolase 4.579E-05 81172.77
Peptides Identified P MW(D a)
K.SPIR.Y 9.457E-01 36.07
K.SFNLSALEK.H 9.684E-01 338.72
R.QVLLSEPEEAAALYR.G 4579E-05 1022.76
  1. LC/MS-MS of the bands excised from an activity stained native electroblot membrane confirmed the presence of OPH. OPH was not consistently identified in band 1. P indicates the probability that the protein or peptide is a random match to the spectral data.