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Table 2 Electroblot esterase activity bands contain OPH

From: Identification of oxidized protein hydrolase as a potential prodrug target in prostate cancer

B and 2

Protein Identified

P

MW(D a)

N-acylaminoa cly-peptide hydrolase

3.710E-05

81172.77

Peptides Identified

P

MW(D a)

R.QVLLSEPEEAAALYR.G

1.230E-02

556.27

K.ALDVSASDDEIAR.L

5.072E-01

103.52

K.SFNLSALEK.H

8.916E-01

159.19

K.AESFFQTK.A

9.150E-01

159.21

R.VVFDSAQR.S

8.716E-01

113.36

R.QVLLSEPEEAAALYR.G

4.985E-05

439.67

B and 3

Protein Identified

P

MW(D a)

N-acylaminoacyl-peptide hydrolase

1.857E-07

81172.77

Peptides Identified

P

MW(D a)

R.QVLLSEPEEAAALYR.G

1.857E-07

1185.89

R.GSTGFGQDSILSLPGNVGHQDVK.D

3.389E-04

541.33

K.TPLLLML GQEDR.R

2.792E-05

1402.32

R.NVPVR.D

9.825E-01

91.84

B and 4

Protein Identified

P

MW(D a)

N-acylaminoacyl-peptide hydrolase

4.579E-05

81172.77

Peptides Identified

P

MW(D a)

K.SPIR.Y

9.457E-01

36.07

K.SFNLSALEK.H

9.684E-01

338.72

R.QVLLSEPEEAAALYR.G

4579E-05

1022.76

  1. LC/MS-MS of the bands excised from an activity stained native electroblot membrane confirmed the presence of OPH. OPH was not consistently identified in band 1. P indicates the probability that the protein or peptide is a random match to the spectral data.