Figure 6

Growth inhibition by 2-FDG or oligo on NB4 and THP-1 under normoxia and hypoxia. NB4 and THP-1 were cultured in the same condition as cell proliferation assay for 48 hours with 2-FDG (0, 2, 5 10 mM) or oligo (0, 0.05, 0.2, 1 μg/ml) in triplicate. The cell numbers after 48 hours culture under normoxia or hypoxia with 2-FDG or oligo were divided by cell numbers of control (average of triplicated experiments) to obtain cell number ratios, in which the cell number with no treatment corresponded to 100%. The growth suppression of THP-1 by 2-FDG even at 2 mM under hypoxia was significantly more pronounced than that under normoxia (p = 0.0005), although the growth inhibition of NB4 by 2-FDG under hypoxia was slightly less than that under normoxia (A). Oligo did not suppress the growth of NB4 under hypoxia, but suppressed that under normoxia (at 1 μg/ml, p < 0.0001). Oligo similarly suppressed the growth of THP-1 under normoxia and hypoxia (B). ▲: under hypoxia, ●: under normoxia. *** p < 0.001, ** p < 0.01, ns p > 0.05 (unpaired, two-tailed t-test).