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Figure 2 | BMC Cancer

Figure 2

From: Adaptation of leukemia cells to hypoxic condition through switching the energy metabolism or avoiding the oxidative stress

Figure 2

Apoptosis assay by annexin V staining under hypoxia versus normoxia on NB4 and THP-1. NB4 and THP-1 were cultured in the same condition as cell proliferation assay for 24 hours in triplicate. Then, apoptotic cells were examined after 12 and 24 hours by annexin V / PI method. Apoptotic cells of NB4 (A) under 24 hours hypoxia were significantly increased (p = 0.0031), although THP-1 (B) did not show increased apoptosis under hypoxia at least 24 hours (p = 0.7876). ▲: under hypoxia, : under normoxia. H: under hypoxia, N: under normoxia. *** p < 0.001, ns p > 0.05 (unpaired, two-tailed t-test).

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