Figure 1From: Adaptation of leukemia cells to hypoxic condition through switching the energy metabolism or avoiding the oxidative stress Cell proliferation of four leukemia cell lines with no treatment under normoxia or hypoxia. 2 × 105/ml cells in 0.5 ml RPMI1640 + 3% FCS were cultured under normoxia (21% O2) or hypoxia (1% O2) for 48 hours in triplicate. Then, cell count was measured after 24 and 48 hours, respectively. NB4 (A) significantly diminished their proliferation under hypoxia compared with normoxia (48 hours) (p = 0.0001). In contrast, THP-1 (B) significantly increased more under hypoxia than normoxia (48 hours) (p = 0.005). Kasumi-1 (C) and HL-60 (D) did not show significant difference of cell growth between normoxia and hypoxia (48 hours). ▲: under hypoxia, ●: under normoxia. *** p < 0.001, ** p < 0.01, ns p > 0.05 (unpaired, two-tailed t-test).Back to article page