Figure 5
ALDH1 expression in clinical specimens of sarcoma patients treated with preoperative sorafenib or pazopanib prior to surgical resection. A. Increases in ALDH1 staining intensity among soft tissue sarcoma patients treated with neoadjuvant sorafenib and conformal radiotherapy on a Phase I trial (Clinical Trial Information: NCT#00805727/UCDCC#216). B. Representative photomicrographs of ALDH1 expression from a patient pre- (top) and post-treatment (bottom) evaluated on tissue microarray. Three cores per patient were analyzed. Boxed areas are depicted at higher magnification. C. Negligible effects in ALDH1 staining intensity among soft tissue sarcoma patients sarcoma ineligible for NCT#00805727/UCDCC#216 who underwent diagnostic biopsy followed by definitive surgical resection without preoperative therapy. D. Representative photomicrographs of ALDH1 expression from a patient pre- (top) and post-treatment (bottom) from a tissue microarray created from formalin-fixed, paraffin-embedded specimens of patients with soft tissue sarcoma treated with a biopsy followed by definitive surgical resection without preoperative therapy. Three cores per patient were analyzed. Boxed areas are depicted at higher magnification. E. High power view shows high grade undifferentiated pleomorphic sarcoma from patient SA-0751 treated clinically with pazopanib for metastatic sarcoma. (Middle) Flow cytometry reveals no significant difference in ALDHbright cells from fresh tissue evaluated before and after pazopanib. (Right) Representative flow cytometry plots of ALDH expression are shown. ALDH scores before and after treatment were analyzed using the two-sided paired t-test. All experiments were performed in triplicate.