Figure 3

Dose-dependent cell cycle perturbation in Type II EC cell lines with HCI2509 treatment. (A, B) Cell cycle populations of (A) AN3CA and (B) KLE cell lines after exposure to vehicle, 300 nM, 1 μM, and 3 μM HCI2509 for 48 hours. 2 × 10⁴ counts and 1 × 10⁴ counts were used for AN3CA and KLE cells, respectively. Data is representative of four biological replicates. Mean and standard deviation are plotted (* p < 0.05, ** p < 0.01).