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Figure 2 | BMC Cancer

Figure 2

From: Reversible inhibition of lysine specific demethylase 1 is a novel anti-tumor strategy for poorly differentiated endometrial carcinoma

Figure 2

Treatment with HCI2509 causes changes in global histone methylation and induces LSD1 target genes. (A, B) Western blot analysis of H3K4me3, H3K9me2, H3K27me3 and LSD1 after 48 hours of vehicle or HCI2509 treatment at varying concentrations in (A) AN3CA and (B) KLE cells. Images are representative of two repeat experiments performed in triplicate. (C, D) qRT-PCR analysis of LSD1 target genes, HMOX1 and CDH1, after treatment with 3X EC50 for (C) AN3CA and (D) KLE cells. Data represents the mean and standard deviation (n = 3) and all replicates were normalized to internal housekeeping gene RPL19.

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