Less tumor EAG1 protein expression was found in mice treated with the combined treatment. A) Representative Western blot of EAG1 expression showing two different T-47D tumors per treatment. The mice bearing palpable tumors received vehicle (Vh); calcitriol (C), astemizole (A) or the combination of both drugs (CA) for 3 weeks. Tumors were excised, homogenized and 30 μg of total protein were loaded per lane. Two immunoreactive bands of ~110 kDa and 130 kDa (indicated by arrows) were detected. B) and C) represent the quantification of the 130 kDa and 110 kDa bands, respectively. The expression of each of the two species of EAG1 was normalized against GAPDH optical density (O.D) and results are shown as the mean ± standard deviation of EAG1 relative O.D of four different tumors from each treatment. *P < 0.05 vs. Vh. Vehicle was given an arbitrary value of 1.