The combined treatment with calcitriol and astemizole downregulated EAG1 gene expression in the tumoral tissue. The gene expression of tumoral EAG1 was assessed by RT-qPCR and normalized against the corresponding housekeeping gene. Values for controls were arbitrarily set to one. Black bars = MBCDF; white bars = T-47D. *P < 0.05 vs. control in each group; n ≥ 15. Results are expressed as the mean ± standard error.