Figure 3

Ki-67 was expressed by less tumoral cells in mice with combined therapy compared to monotherapy. Ki-67-positive cells in the tumors were counted in slides from the groups of mice treated with: vehicle (Vh), calcitriol (C), astemizole (A) or their combination (CA). Upper panel: Representative pictures of T-47D tumors from mice treated with the different drugs, showing immunohistochemistry studies of Ki-67 (nuclear, brown staining). Inset shows negative control in the absence of first antibody. All pictures are 20 X. Lower panel: Graphical representation of positive Ki-67 cells in immunohistochemistry slides (%). Black bars = MBCDF; white bars = T-47D. After identifying areas with the most intensive staining, counting of total cells was done in three different fields per tumor slide and the percentage of Ki-67- positive cells was calculated. Results are expressed as the mean ± standard error. * P < 0.05 vs vehicle in each group. **P < 0.05 vs each drug alone.