T-47D and MBCDF cell lines express VDR and EAG1. The cell lines used in this study T-47D (A-C) and MBCDF (D-F) express both biomarkers for the targeted therapy. Cells were incubated in the presence of anti-VDR (A, D), anti-EAG1 (B, E) or without first antibody (C, F) as negative control (C-) and further processed as described under Materials and Methods. Positive immunoreactivity is shown in brown staining. Representative pictures are shown (40 X).