Characterization of subcutaneous xenograft model of VCaP-Luc. (A) Tumor growth curves of VCaP-Luc cells subcutaneously implanted in intact (sham surgery), castrated (castrated before tumor implantation) and intact-castrated mice (castrated 5 weeks after tumor implantation, as indicated by the arrow). Error bar represents SEM of n = 10 mice/group. (B) Western blot of androgen receptor expression in VCaP-Luc subcutaneous tumors grown in intact (I), castrated (C) and intact-castrated (I-C) mice. Tumors were harvested at approximately 800 mm3. Representative image from 3 tumor samples.