Figure 6

MFE (%) decreases upon siRNA-mediated silencing of CDH3 , HIF-1α, GLUT1 and CAIX in SUM149 cells. siRNA-mediated silencing of CDH3, HIF-1α, GLUT1 and CAIX was confirmed by western blot analysis (A). A statistically significant decrease in MFE (%) was observed when we silenced CDH3, HIF-1α, GLUT1 and CAIX. The simultaneous silencing of CDH3 and HIF-1α also revealed a significant decrease of MFE (%) of the target cells, although not cumulative. Still, the silencing of the expression of all transcripts tested led to a non-cumulative inhibition of the MFE compared to the control cells (B). *p < 0.05.