Figure 4

P-cadherin expression affects GLUT1 and CAIX mRNA levels in breast cancer cells. mRNA expression measured by qRT-PCR of CDH3, HIF-1α, GLUT1, CAIX, MCT1 and CD147 when inducing siRNA-mediated knockdown of CDH3 , GLUT1 and CAIX in BT20 and SUM149 breast cancer cell lines. Upon CDH3 silencing, there was a significant decrease of the mRNA expression of GLUT1 and CAIX in BT20 (A) and a tendency, although not statistically significant, to a decrease in SUM149 cells (D). No differences were observed in the mRNA expression of HIF-1α, MCT1 and CD147 in both cell lines. On the other hand, there were no alterations in CDH3 mRNA expression in BT20 (B and C) and in SUM149 (E and F) breast cancer cell lines, when GLUT1 (B and E) and CAIX (C and F) were silenced. *p < 0.05; **p < 0.0001.