HIF-1α stabilization induces the expression of P-cadherin in the membrane of SUM149 breast cancer cells. HIF-1α stabilization and accumulation by CoCl2 treatment was confirmed by western blot (A). qRT-PCR showed no significant alterations (p = 0.562) in CDH3 mRNA levels upon CoCl2 treatment (B). Using FACS analysis, a statistically significant increase (p = 0.0246) was observed in the expression of membrane P-cadherin in CoCl2 treated cells, when compared with the control cells treated with the vehicle (EtOH) (C). Immunofluorescence of CoCl2 treated cells showed nuclear HIF-1α expression (red) and an increase of membrane P-cadherin expression (green), compared with vehicle treated cells (D). Z-stack measurements revealed a decrease in the height of CoCl2-treated cells comparing with the control cells (E). *p < 0.05.