| |
ALG 1: percentage fraction of the COX-2 positive cells (0–100) with a mean value of all the results used for recognition of tumour overexpressing the COX-2 protein
|
ALG 2: percentage fraction of the COX-2 positive cells (0–100) with a cut-off of 10% used for recognition of tumour overexpressing the COX-2 protein
|
ALG 3: percentage fraction of the COX-2 positive cells (0–100) × staining intensity (1–3) with a median value of all the results used for recognition of tumour overexpressing the COX-2 protein
|
|---|
|
Anti-COX-2 antibody
|
Ab1
|
Ab2
|
Ab3
|
Ab1
|
Ab2
|
Ab3
|
Ab1
|
Ab2
|
Ab3
|
|
Type of the cells scored (E – epithelial; S – stromal)
|
E
|
S
|
E
|
S
|
E
|
S
|
E
|
S
|
E
|
S
|
E
|
S
|
E
|
S
|
E
|
S
|
E
|
S
|
|
Cut-off value
|
5.1
|
4.2
|
21.1
|
9.8
|
43.0
|
19.0
|
10% of the cells with a moderate or strong staining
|
6.2
|
8.2
|
27.3
|
15.3
|
68.0
|
30.1
|
|
Percentage fraction of the tumours overexpressing COX-2
|
41.5
|
41.5
|
51.2
|
39.0
|
46.3
|
48.8
|
0
|
2.4
|
26.8
|
29.3
|
26.8
|
80.5
|
48.8
|
48.8
|
48.8
|
48.8
|
48.8
|
46.3
|
- Staining was evaluated with three different algorithms (ALG1, ALG2 and ALG3) applied to data obtained using three different anti-COX-2 antibodies (Ab1, Ab2 and Ab3).
