The secondary structure and titration curve of the binding of metal complexes to the apo-form of the BRCA1 protein (without Zn
). (A) The effect of the ruthenium(II) complexes on the secondary structures of proteins was predicted by the CONTIN program. The relative secondary structure of the Ru-BRCA1 adducts at 50 μM of the complexes were plotted. (B) Changes in the ellipticity of the protein at 208 nm with increasing metal concentrations were plotted.