Figure 10
CD spectra of ruthenium-BRCA1 adducts. Ten μM of BRCA1 protein was pre-incubated with 30 μM of ZnCl2 at 4°C for 8 h. Samples were then incubated with the ruthenium(II) complexes in the dark at ambient temperature for 16 h before CD measurement at 25°C with a scanning rate of 50 nm/min. The mean residues ellipticity and wavelength that ranged from 200 to 260 nm were plotted. The CD spectra of the 1-BRCA1 adducts (A), 2-BRCA1 adducts (B) at a number of concentrations that induced secondary structure changes of the holo-form of the BRCA1 protein.