Figure 9
From: Nitidine chloride inhibits hepatic cancer growth via modulation of multiple signaling pathways
Effect of NC on the activation of SHH pathway in hepatic cancer xenograft mice and HepG2 cells. (A) Tumor tissues were processed for IHC for SHH and Gli-1. The photographs are representative images taken at a magnification of 400 ×. Quantification of IHC assay was represented as percentage of positively-stained cells. Data shown are averages with S.D. (error bars) from 6 individual mouse in each group. *P < 0.05; **P < 0.01, versus controls. (B) The protein expression levels of Shh and Gli-1 in HepG2 cells were determined by Western Blotting. β-actin was used as the internal control. The mRNA levels of SHH and Gli-1 in tumor tissues (C) and HepG2 cells (D) were determined by RT-PCR. GAPDH was used as the internal control. Images of Western Blotting and RT-PCR are representatives of 3 individual mouse in each group.