Effect of NC on activation of STAT3 and ERK in hepatic cancer xenograft mice and HepG2 cells. The level of STAT3 or ERK phosphorylation in tumor tissues (A) and ERK phosphorylation in HepG2 cells (B) was determined by Western blot. β-actin was used as the internal control. Data panels from A-B are representatives of 3 individual mouse in each group. (C) IL-6-mediated STAT3 transcriptional activity in HepG2 cells. The transcriptional activity of STAT3 was measured by DLR assay. Data are averages with S.D. (error bars) from at least three independent experiments. *P < 0.05, versus control cells; #
P < 0.05, versus cells treated with IL-6 but without NC.