Effect of NC on the expression of Cyclin D1 and CDK4 in hepatic cancer xenograft mice and HepG2 cells. The mRNA levels of Cyclin D1 and CDK4 in tumor tissues (A) or HepG2 cells (B) were determined by RT-PCR. The protein expression levels of Cyclin D1 and CDK4 in tumor tissues (C) or HepG2 cells (D) were determined by Western blotting. GAPDH and β-actin were used as the internal controls for the RT-PCR or Western blotting, respectively. Images are representatives of 3 individual mouse in each group or of three independent cell-based experiments.