Immunohistochemical analysis of Ep-ICD expression in breast cancer. Representative photomicrographs demonstrating: (I) predominantly cytoplasmic Ep-ICD expression in normal breast tissues. Nuclear and cytoplasmic accumulation of Ep-ICD in: (II) DCIS; (III) IDC; (IV) ILC; (V) IMC; and (VI) negative control breast cancer tissue incubated with isotype specific IgG showing no detectable immunostaining for Ep-ICD. The arrows labelled N and C depict nuclear, and cytoplasmic staining respectively. (original magnification × 400).