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Figure 8 | BMC Cancer

Figure 8

From: Differential modulation of nicotine-induced gemcitabine resistance by GABA receptor agonists in pancreatic cancer cell xenografts and in vitro

Figure 8

A: Western blots of signaling proteins in PANC-1 cells in vitro. Exposure to single doses or 7 days of GABA (50 μM), nicotine (1 μM) or the combination of both agents modulated the protein expression of the metalloproteinases MMP-2 and MMP-9 and on the transcription factor EGR-1 in PANC-1 cells in vitro. B: Densitometry data from PANC-1 cells treated for 7 days. Quantitative densitometry was generated from triplicate western blots of PANC-1 cells after 7 days of treatment. The differences among treatment groups for MMP-2, MMP-9 and EGR-1 were significant (p < 0.0001 by Kruskal-Wallis ANOVA). Nicotine induced the expression of these three proteins while GABA had strong inhibiting effects in the absence and presence of nicotine. Significantly ( p < 0.005 by Mann–Whitney test) different from controls. Significantly ( < 0.005 by Mann–Whitney test) different from treatment with nicotine. C: Quantitative densitometry data after single dose treatments of PANC-1 cells. The differences among treatment groups for MMP-2, MMP-9 and EGR-1 were significant (p < 0.0001 by Kruskal-Wallis ANOVA). Nicotine induced the expression of these three proteins while GABA had strong inhibiting effects in the absence and presence of nicotine. Significantly ( p < 0.005 by Mann–Whitney test) different from controls. Significantly ( < 0.005 by Mann–Whitney test) different from treatment with nicotine.

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