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Figure 7 | BMC Cancer

Figure 7

From: Curcumin restores sensitivity to retinoic acid in triple negative breast cancer cells

Figure 7

Curcumin and ATRA reduce VEGF-A expression in MDA-MB-231 cells. (A) MDA-MB-231 cells were plated in 96 well plates treated with 1 μM ATRA (RA), 30 μM curcumin (C) or 30 μM curcumin and 1 μM ATRA for 48 hours. The control was DMSO, ethanol or a combination of both. The % BrdU incorporation for each of the treatment (curcumin, ATRA or both) was calculated relative to their solvent, DMSO, ethanol or DMSO and ethanol, respectively. Data are mean of ± SE (n = 3). *, p = 0.035 versus curcumin. (B) MDA-MB-231 cells were treated with 30 μM curcumin (C) for 24 hours prior to the addition of 1 μM ATRA (RA) for 4 hours. Total RNA was collected from MDA-MB-231, qRT-PCR was performed and expression levels of PDK1 were measured. GAPDH was used for normalization. Data are mean of ± SE (n = 3). *, p = 0.012 versus curcumin. (C) MDA-MB-231 cells were plated in 96 well plates treated with 1 μM ATRA for 2–8 days. The % BrdU incorporation for each of the treatment was calculated relative to their solvent, DMSO. Data are mean of ± SE (n = 3). *, p = 0.0008 versus control ** p = 0.002 versus control. (D) MDA-MB-231 cells were treated with 30 μM curcumin (C) for 24 hours prior to the addition of 1 μM ATRA (RA) for 4 hours. Total RNA was collected from MDA-MB-231, qRT-PCR was performed and expression levels of VEGF-A was measured. GAPDH was used for normalization. Data are mean of ± SE (n = 3). +, p = 0.016 versus control, ++, p = 0.03 versus curcumin.

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