Table 1 Primer sequences used for RT-PCR and Q-PCR in this study
From: Implication of metastasis suppressor gene, Kiss-1 and its receptor Kiss-1R in colorectal cancer
Genes | Sense (5'-'3) | Antisense (5'-'3) | |
|---|---|---|---|
Kiss-1 | Conventional PCR | TGAACTCACTGGTTTCTTGG | CGAAGGAGTTCCAGTTGTAG |
Quantitative PCR | CATTAGAAAAGGTGGCCTCT | ACTGAACCTGACCGTACAGCCCAGGGATTCTAGCTG | |
Anti-Kiss1 ribozyme1 | CTGCAGCTCTCGGGGGGCGGGGACAGCGAGGTCCCCCCTGATGAGTCCGTGAGGA | ACTAGTGCCAGCTGCTACTGCCAGGCTGAGCCGTTTCGTCCTCACGGACT | |
Anti-Kiss-1 ribozyme2 | CTGCAGCACCGCGCCCTGGGGTGCGGGCTGATGAGTCCGTGAGGA | ACTAGTGTCCGCCCCCCACAGCCGCCAGATTTCGTCCTCACGGACT | |
Kiss-1R | Conventional PCR | CTTCATGTGCAAGTTCGTC | CACCAGGAACAGCTGGAT |
Quantitative PCR | GCTGGTCATCTACGTCATCT | ACTGAACCTGACCGTACACAGCACAGGAGGAAGGTC | |
Anti-Kiss-1R ribozyme1 | CTGCAGTTCCGCATCGGCTTGTGGCGGCACTGATGAGTCCGTGAGGA | ACTAGTTCGCTGGTCATCTACGTCATTTCGTCCTCACGGACT | |
Anti-Kiss-1R ribozyme2 | CTGCAGAGCCTACCCAGATGCTGAGGCTCTGATGAGTCCGTGAGGA | ACTAGTGCCCCGCCTGGCGCTGGCTGTTTCGTCCTCACGGACT | |
GAPDH | Conventional PCR | GGCTGCTTTTAACTCTGGTA | GACTGTGGTCATGAGTCCTT |
| Â | Quantitative PCR | CTGAGTACGTCGTGGAGTC | ACTGAACCTGACCGTACACAGAGATGATGACCCTTTTG |