Knockdown of Kiss-1 altered functions of colorectal cancer cells. A. RT-PCR displayed a reduced mRNA expression level of Kiss-1 and Kiss-1R in HT115/ HRT18 Kiss-1 knockdown cells and HT115/ HRT18 Kiss-1R knockdown cells compared with control cells (WT and pEF) respectively. Placenta was used as a positive control for both Kiss-1 and Kiss-1R (far right panel). B. Left: The influence of Kiss-1 and Kiss-1R knockdown on the invasive capability of HT115 cells (left) and HRT18 cells (right). Three representative images of cells following staining are shown in the top. Data shown is the mean value of 3 repeats of experiment. C. The effect of Kiss-1 and Kiss-1R knockdown on the migration ability of HT115 cells (left) and HRT18 cells (right). Data shown is the mean value of 3 repeats of experiment. Error bars represent standard deviation. * stands for p < 0.05.