Figure 5

Flow cytometric analysis of CD24, CD90, and CD133 expressions. Cells were stained with APC-conjugated anti-CD24 antibody ( A ), FITC-conjugated anti-CD90 antibody ( B ), and PE-conjugated anti-CD133 antibody ( C ) and then separated with a flow cytometer. Sphere cells showed a decreased population of CD24 and CD90 positive cells than their parental cells. SK-HEP-1, HLE, and these derivative cells were negative for CD133.