Figure 4

miRNA expressions were restored after RNA interference study. (A) Relative expressions of miR-21, miR-222 and miR-145 in HepG2 cells by co-transfection with HBx plasmid (0.8 μg) and HBx–siRNA (40 p mole) or negative control (NC). RNA were collected 24 and 48 h after each transfection. (B) Relative expressions of miR-21, miR-222 and miR-145 were measured when HepG2 cells were co-transfected with 1.3 fold full length HBV genome (0.8 μg) and HBx - siRNA (40 p mole) or negative control. RNA were collected 24, 48 and 72 h post transfection. (C) HepG2.2.15 cells were transfected with HBx siRNA (80 p mole) or negative control (NC) and relative expressions of miR-21, miR-222 and miR-145 were measured. RNA were collected 24, 48 and 72 h post transfection. Data plotted are the mean ± SD normalized to U6 expression. The experiments were performed in triplicate (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test).