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Figure 2 | BMC Cancer

Figure 2

From: Tumor suppressor micro RNA miR-145 and onco micro RNAs miR-21 and miR-222 expressions are differentially modulated by Hepatitis B virus X protein in malignant hepatocytes

Figure 2

Expressions of target mRNAs in HBx and HBV transfected and constitutively HBV synthesizing hepatoblastoma cells. (A) Relative expressions of PTEN, p27 and MAP3K (Raf 1) - targets of miR-21, miR-222 and miR-145 respectively in HBx transfected HepG2 cells. Cells were transfected with 1 μg of pCXN2-HBx or pCXN2 as a control. (B) Relative expressions of PTEN, p27 and MAP3K (Raf 1) in 1.3 fold full length HBV genome transfected HepG2 cells. Cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 control vector. (C) Relative expressions of PTEN, p27 and MAP3K (Raf 1) in HepG2.2.15 cell line. RNA were extracted 48 hours post transfection. The mRNA expressions were measured by qRT-PCR and the expressions were normalized to GAPDH. Data are expressed as the mean ± SD from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test).

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BMC Cancer

ISSN: 1471-2407

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