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Figure 1 | BMC Cancer

Figure 1

From: Tumor suppressor micro RNA miR-145 and onco micro RNAs miR-21 and miR-222 expressions are differentially modulated by Hepatitis B virus X protein in malignant hepatocytes

Figure 1

HBx modulates the expressions of miR-21, miR-222 and miR-145 in hepatoblastoma cells in vitro. (A) The relative expressions of miR-21, miR-222 and miR-145 in HepG2 cells transiently transfected with HBx expressing plasmid pCXN2-HBx or control vector. Cells were transfected with 1 μg of pCXN2-HBx or pCXN2 as a control. (B) The relative expressions of miR-21, miR-222 and miR-145 in HepG2 cells transiently transfected with 1.3 fold full length HBV genome cloned into pUC19 plasmid or control vector. The cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19. (C) The relative expressions of miR-21, miR-222 and miR-145 in stably HBV producing HepG2.2.15 cell line or control HepG2 cells. Cells were collected for analysis 48 hour after each transfection. The miRNA expressions were measured by qRT-PCR. Plotted are the mean ± SD of three samples normalized to U6 expression (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test).

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BMC Cancer

ISSN: 1471-2407

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