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Figure 5 | BMC Cancer

Figure 5

From: Mesenchymal stem cells are efficiently transduced with adenoviruses bearing type 35-derived fibers and the transduced cells with the IL-28A gene produces cytotoxicity to lung carcinoma cells co-cultured

Figure 5

Growth inhibition of MSCs transduced with AdF35-IL-28. (A) Expression of IL-28 receptor complexes in human fibroblasts and MSCs. RT-PCR showed IL-28Rα and IL-10β gene products. TE-11 cells were used as a positive control for the gene expression and GAPDH as a loading control. (B) MSCs (100 or 300/well), uninfected or infected with AdF35-IL-28A or AdF35-LacZ (MOI = 100), were cultured alone or with OBA-LK1 cells (1,000/well) for 4 days. Viability of each group was detected with the WST-8 reagent and expressed as absorbance at 450 nm. SE bars are also shown (n = 3). *P < 0.05 and #P < 0.01, comparing MSCs infected with AdF35-IL-28A versus MSCs uninfected or infected with AdF35-LacZ.

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BMC Cancer

ISSN: 1471-2407

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