PDA-66 does not influence expression of proteins of Wnt/β-catenin pathway but alters expression of 4EBP-1. After treatment with PDA-66 and DMSO, respectively, cells were lyzed and protein expression analyzed with Western blot. (A) Exemplary results of PDA-66 treated SEM cells after 4 and 24 h are displayed. No influence on expression of total GSK3β and the total form of Akt could be noticed. PhosphoGSK3βSer9 seemed decreased at higher PDA-66 concentrations after 4 h. No influence on the amount of β-catenin was observed. (B) Exemplary results of SEM, RS4;11 and MOLT4 cells are displayed. In SEM and RS4;11 cells a decrease of 4EBP-1 and p4EBP-1Ser65 was detectable, in contrast MOLT4 cells showed an increased expression of p4EBP-1Ser65 after PDA-66 treatment.