Figure 2
From: A novel deleterious PTEN mutation in a patient with early-onset bilateral breast cancer
Genetic and molecular characterization of PTEN c.71A>T mutation. A) Electropherogram showing PTEN c.71A > T transversion; B) PTEN amino acidic sequence alignment among species: H.sapiens [HS], P.troglodytes [PT], M.mulatta [MM], C.lupus [CLP], B.taurus [BT], M.musculus [MusM], R.norvegicus [RN], G.gallus [GG], X.laevis [XL], D.rerio [DR], D.melanogaster [DM] and A.gambiae [AG]; C) Western Blot analysis on total cell lysates of XTC.UC1 cells tranfected with wild type PTEN [WT], PTENAsp24Val, PTENAsn48Lys, on Empty Vector [EV], and on untransfected XTC.UC1 cells, showing that only PTEN WT is able to inhibit P-AktSer473, P-AktThr308 and P-S6RP; D) Immunohistochemical analyses of the breast tumors [Magnification 200X]: Phospho-AKT-Ser473 immunostaining, showing negative reaction in normal breast duct (a), strong nuclear and cytoplasmic immunoreactivity in infiltrating (b) and in situ (c) breast tumors; PTEN immunostaining, showing normal reaction in normal duct (d) and in breast infiltrating and in situ carcinomas (e, f).