Figure 3From: Inhibition of long non-coding RNA NEAT1 impairs myeloid differentiation in acute promyelocytic leukemia cells Knocking down NEAT1 impairs neutrophil differentiation in APL cells. (A) 48 hrs after transfection, the knockdown efficiency was confirmed by qRT-PCR. (B) CEBPB mRNA levels were measured by qRT–PCR and are given as n-fold changes compared with untreated cells and normalized to the housekeeping gene ACTB. (C) Flow cytometry analysis of ITGAM/CD11b surface expression of control and si-NEAT1 cells upon 48 h of ATRA-treatment (1 μM). CD11b expression was measured by flow cytometry and values were normalized to untreated control cells. Data are shown as the mean ± SD of three separate experiments.Back to article page