The lncRNA NEAT1 is significantly down-regulated in APL primary patient samples. (A) The NEAT1 isoforms are shown schematically. The black boxes indicate the position of sequences amplified by qRT-PCR. (B) Comparison of NEAT1 expression in granulocytes from healthy donors (Granulo, n =12) compared with primary APL cells (n =31). The expression levels of the NEAT1 isoforms were evaluated by qRT-PCR. Malat1 lncRNA served as negative control and shown in Additional file 1: Figure S1A. Measured cycle threshold (Ct) values represent log2 expression values. The values were normalized to the expression level of the housekeeping gene ACTB. Each data point represents 1 patient sample.