Pathological and immunohistochemical characterisation of tumors. Pathologic analyses were performed on paraffin-embedded tissue blocks. Haematoxylin and eosin staining (HES) confirmed invasive SBR3 carcinomas. No staining was detected after estrogen receptor (1D5, DAKO) and progesterone receptor (PgR 636 DAKO) branding compared to positive and negative controls. Using the A485 polyclonal antibody (DAKO), a strong brown complete membrane staining was detected in more than 30% tumor cells in both patients.