Skip to main content

Advertisement

Table 1 Primary antibodies used in the immunohistochemistry

From: Immunohistochemical analysis of cancer stem cell markers in pancreatic adenocarcinoma patients after neoadjuvant chemoradiotherapy

Antigen (clone) Location Antibody species Manufacturer (product) Antigen-retrieval solution Dilution Internal positive control [reference No]
EpCAM (ESA) M Mouse monoclonal Dako (M3525) PH6 ciltrate buffer 1:200 Epithelium of pancreatic ducts, acinar cells and islets of Langerhans cells [29]
CD24 M Mouse monoclonal Neomarkers (MS-1279) PH6 ciltrate buffer 1:50 Acinar cells [27]
CD44 M Mouse monoclonal Abcam(ab51037) PH6 ciltrate buffer 1:50 Acinar cells [28]
CD133 M Rabbit polyclonal Abnova(12663) PH9 Tris EDTA buffer 1:50 Acinar cells [19]
CXCR4 M Mouse monoclonal Zymed(35-8800) PH6 ciltrate buffer 1:50 Acinar cells [17]
ALDH1 C Mouse monoclonal Abcam(ab52492) PH6 ciltrate buffer 1:100 Acinar cells and islets of Langerhans cells [19]
  1. M: membrane C: cytoplasm.