Table 1 Primary antibodies used in the immunohistochemistry
Antigen (clone) | Location | Antibody species | Manufacturer (product) | Antigen-retrieval solution | Dilution | Internal positive control [reference No] |
|---|---|---|---|---|---|---|
EpCAM (ESA) | M | Mouse monoclonal | Dako (M3525) | PH6 ciltrate buffer | 1:200 | Epithelium of pancreatic ducts, acinar cells and islets of Langerhans cells [29] |
CD24 | M | Mouse monoclonal | Neomarkers (MS-1279) | PH6 ciltrate buffer | 1:50 | Acinar cells [27] |
CD44 | M | Mouse monoclonal | Abcam(ab51037) | PH6 ciltrate buffer | 1:50 | Acinar cells [28] |
CD133 | M | Rabbit polyclonal | Abnova(12663) | PH9 Tris EDTA buffer | 1:50 | Acinar cells [19] |
CXCR4 | M | Mouse monoclonal | Zymed(35-8800) | PH6 ciltrate buffer | 1:50 | Acinar cells [17] |
ALDH1 | C | Mouse monoclonal | Abcam(ab52492) | PH6 ciltrate buffer | 1:100 | Acinar cells and islets of Langerhans cells [19] |