Figure 6
From: A-770041 reverses paclitaxel and doxorubicin resistance in osteosarcoma cells
Fluorescent images of dose-dependent increase in intracellular accumulation of calcein AM in KHOS R2 following 30 min treatment with A-770041. The calcein AM assay was optimized and performed using the Vybrant Multidrug Resistance kit in KHOSR2 cells. Cells were plated onto 96-well plates at a density of 4 × 103 cells/well in a volume of 100 μl RPMI1640 medium and grown for 24 h. Different concentrations (0, 0.01, 0.1 and 0.5 μM) of A-770041 were added for one hour. 50 μl of calcein AM were added to each well. After 30 minutes exposure to calcein AM at 37°C, cells were counterstained with Hoechst 33342 for 2 min. The cell fluorescence images were acquired by a fluorescence microscope.