Figure 2

Regulation of miR-27a in the proliferation and apoptosis in the Hep2 cells. (A) Transfection efficiency of miR-27a in the Hep2 cells observed by fluorescence microscope. miR-27 labeled with FAM was transfected into the Hep2 cells and the fluorescence protein at 6 hr after transfection was detected using a fluorescence microscope. (B) Transfection efficiency of the corresponding miRNAs in the Hep2 cells by qRT-PCR. After transfection, the expression of miR-27a or the control miRNAs in the Hep2 cells were monitored using qRT-PCR. (C) Effect of miR-27a on the Hep2 cell proliferation measured by the MTT assay. Hep2 cells were transfected with miR-27a or the control miRNAs in the Hep2 cells and the cell proliferation was detected using the MTT assay. (D) Effect of miR-27a on the Hep2 cell proliferation measured by the colony formation assay. Hep2 cells were transfected with miR-27a or the control miRNAs in the Hep2 cells and the cell proliferation was detected using the colony formation assay. (E) Effect of miR-27a on the early apoptosis of the Hep2 cell lines. Hep2 cells were transfected with miR-27a or the control miRNAs and treated by Annexin V-EGFP apoptosis detection kit. The early apoptotic percentages of the Hep2 cells in different groups were monitored by flow cytometry. (F) Effect of miR-27a on the late apoptosis of the Hep2 cell lines. Hep2 cells were transfected with miR-27a or the control miRNAs and treated by Annexin V-EGFP apoptosis detection kit. The late apoptotic percentages of the Hep2 cells in different groups were monitored by flow cytometry. Data were expressed as the mean ± SD from three independent experiments. P < 0.05 is indicated as symbol*.