Figure 4

Fibulin-1 inhibited motility and angiogenesis activation of bladder cancer cells. A) 5637 and C) HT1376 cells were transfected with pEGFP-N1 or pEGFP-FBLN1 for 72 h and then the abilities of cell migration and invasion were detected respectively. Representative images showed results of one assay, B) and D) the column chart showed the mean ± SD of five randomly selected microscope fields per well. E) Tube formation of HUVECs was determined by assaying the numbers of branch nodes after 24 h of culture under a phase contrast microscope. HUVECs were cultured in the following media: conditioned media (CM) of 5637 cells, CM of 5637 cells transfected with pEGFP-N1, CM of 5637 cells transfected with pEGFP-FBLN1 and CM of 5637 cells transfected with pEGFP-FBLN1 with fibulin-1 antibody pretreatment. F) The summary and statically analysis of E). All these experiments were repeated at least three times. Asterisk indicates p < 0.05.