Fibulin-1 was down-regulated in bladder cancer. A) Fibulin-1 mRNA and B) protein expression levels were evaluated by qPCR and Western blot assay respectively in bladder cancer cell lines. GAPDH served as an internal control and loading control. C) Representative microphotographs of fibulin-1 staining in patient tissues. D) Summary of fibulin-1 expression determined by immunohistochemistry in NMIBC patient tissue samples. E) Kaplan-Meier estimate of recurrence-free survival stratified by fibulin-1 expression in 139 NMIBC patients.