Figure 4

N-acetylalaninate prodrugs are activated by OPH with a preference for NPAA. A) A reaction mixture containing reduced GSH, S-NPAA, and the indicated treatment was incubated with or without active human OPH in a 96 well plate. Diisopropyl fluorophosphate (DFP) is an irreversible serine protease inhibitor. At each time point, the amount of reduced GSH was measured as described in the Methods Section. B) A reaction mixture containing reduced GSH and the indicated prodrug was incubated with active human OPH in a 96 well plate. At each time point, the amount of reduced GSH was measured as in A). Data points marked with letters that are not the same are significantly different at p < 0.05.