Skip to main content

Advertisement

Figure 4 | BMC Cancer

Figure 4

From: In vitro evaluation of novel N-acetylalaninate prodrugs that selectively induce apoptosis in prostate cancer cells

Figure 4

N-acetylalaninate prodrugs are activated by OPH with a preference for NPAA. A) A reaction mixture containing reduced GSH, S-NPAA, and the indicated treatment was incubated with or without active human OPH in a 96 well plate. Diisopropyl fluorophosphate (DFP) is an irreversible serine protease inhibitor. At each time point, the amount of reduced GSH was measured as described in the Methods Section. B) A reaction mixture containing reduced GSH and the indicated prodrug was incubated with active human OPH in a 96 well plate. At each time point, the amount of reduced GSH was measured as in A). Data points marked with letters that are not the same are significantly different at pā€‰<ā€‰0.05.

Back to article page