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Table 2 Results of DNA and cDNA sequencing combined with the results of bacterial subcloning analyses

From: PIN3 duplication may be partially responsible for TP53haploinsufficiency

Number of sample

Diagnosis

TP53mutations

PIN3

Codon 72 (DNA)

Codon 72 (cDNA)

Subcloning results

Type

cDNA

DNA

Colonies

PIN3

Codon 72

Conclusions

1

Glioblastoma

MT1 175;

MT1 > MT2

MT1 = MT2

A1/A2

C/G

C

L1-2

A1

G

A2 MT1

CGC > CAC

Arg > His

MT2 282

CGG > TGG

Arg > Trp

2

Glioblastoma

237; ATG > ATA Met > Ile

MT

WT > MT

A1/A2

C/G

C

L2-2

A1

G

A2 MT

13

Soft tissue sarcoma

216; GTG > ATG Val > Met

MT

MT > WT

A1/A2

C/G

C

L3-1

L3-2

A2

C

A2 MT

20

Colorectal cancer

248; CGG > CAG Arg > Gln

MT

WT > MT

A1/A2

C/G

G

L4-2

A1

C

A2 MT

21

Colorectal cancer

273; CGT > CAT Arg > His

MT > WT

WT > MT

A1/A2

C/G

C

L5-1

L5-2

A2

C

A2 MT

30

Prostate cancer

239; AAC > GAC Asn > Asp

MT

MT = WT

A1/A2

C/G

C

L6-2

A2

C

A2 MT

  1. MT – mutated template; WT – wild-type template; A1, A2 - polymorphic variants of PIN3; C – cytosine; G – guanine.