Skip to main content

Table 2 Results of DNA and cDNA sequencing combined with the results of bacterial subcloning analyses

From: PIN3 duplication may be partially responsible for TP53haploinsufficiency

Number of sample Diagnosis TP53mutations PIN3 Codon 72 (DNA) Codon 72 (cDNA) Subcloning results
Type cDNA DNA Colonies PIN3 Codon 72 Conclusions
1 Glioblastoma MT1 175; MT1 > MT2 MT1 = MT2 A1/A2 C/G C L1-2 A1 G A2 MT1
CGC > CAC
Arg > His
MT2 282
CGG > TGG
Arg > Trp
2 Glioblastoma 237; ATG > ATA Met > Ile MT WT > MT A1/A2 C/G C L2-2 A1 G A2 MT
13 Soft tissue sarcoma 216; GTG > ATG Val > Met MT MT > WT A1/A2 C/G C L3-1
L3-2
A2 C A2 MT
20 Colorectal cancer 248; CGG > CAG Arg > Gln MT WT > MT A1/A2 C/G G L4-2 A1 C A2 MT
21 Colorectal cancer 273; CGT > CAT Arg > His MT > WT WT > MT A1/A2 C/G C L5-1
L5-2
A2 C A2 MT
30 Prostate cancer 239; AAC > GAC Asn > Asp MT MT = WT A1/A2 C/G C L6-2 A2 C A2 MT
  1. MT – mutated template; WT – wild-type template; A1, A2 - polymorphic variants of PIN3; C – cytosine; G – guanine.